Avegene
Life Science has developed and marketed a broad range of reagents
for Academic and Industrial markets including Life Science research,
Genomics, Proteomics as well as molecular diagnosis. All Avegene
Reagents are manufactured under ISO 9001 System for an enhanced
quality and for worry free use.
The
High-Speed Plasmid Mini Kit is designed for rapid isolation
of plasmid or cosmid DNA from 1-4 ml of bacterial cultures.
In the process, the modified alkaline lysis method and RNase
treatment are used to get cleared cell lysate with minimal
genomic DNA and RNA contaminants. In the presence of a
chaotropic salt, the plasmid DNA in the lysate binds to
glass fiber matrix in the spin column. The purified plasmid
DNA is eluted by low salt elution buffer or water. The
protocol does not require DNA phenol extraction and alcohol
precipitation. Typical yields are 10-20 mg DNA for high-copy
number plasmid or 0.5-5 mg for low-copy number plasmid.
Fast
Operation
time < 30 minutes
Safety
No phenol
extraction and alcohol precipitation
Convenient
Purified
plasmid is ready to use for PCR, sequencing, restriction
digestion, DNA labeling, transformation
The Gel / PCR DNA
Fragments Extraction Kit is designed to recover or concentrate DNA
fragments (50 bp - 10 kb) from agarose gel, PCR or other enzymatic
reaction. The method uses a chaotropic salt, guanidine thiocyanante
to dissolve agarose gel and denature enzymes, and than DNA fragments
in chaotropic salt are bonded to glass fiber matrix of the spin
column. After washing off the contaminants, the purified DNA
fragments are eluted by low salt elution buffer or water. Salts,
enzymes and unincorporated nucleotides could be effectively removed
from reaction mixture without phenol extraction and alcohol
precipitation. Typical recoveries are 60-80% for gel extraction and
80-90% for PCR cleanup. The entire procedure can be completed in 20
minutes and the eluted DNA is ready to use in restriction digestion,
ligation, PCR, and sequencing reaction.
- Operation time
< 20 minutes
- No phenol extraction and alcohol precipitation
- Purified DNA fragments are ready to use for PCR, sequencing,
restriction digestion, DNA labeling,
ligation
- High recovery, low elution volume
Blood Genomic DNA
Kit is especially designed for purification of total DNA (including
genomic, mitochondrial and viral DNA) from different volume of blood
samples. In this procedure, RBC Lysis Buffer is used to remove
non-nucleated red blood cells and reduce hemoglobin contamination.
Detergents and a chaotropic salt, guanidine hydrochloride can lyse
cells and denature protein, than DNA in chaotropic salt can be bound
to glass fiber matrix of column. After washing off the contaminants,
the purified DNA is eluted by low salt elution buffer or water. The
entire procedure can be completed in 30 minutes without
phenol/chloroform extraction and alcohol precipitation. Purified DNA
with approximately 20-30 kb of size is suitable for PCR or other
enzymatic reactions.
Genomic DNA Kit
for Tissue
Genomic DNA Mini
Kit (Tissue) is especially designed for purification of total DNA
(including genomic, mitochondrial and viral DNA) from a variety of
animal tissues or cells. Provided micropestle can efficiently
homogenize tissue sample to shorten the time on lysis step. The
method use proteinase K and a chaotropic salt, guanidine
hydrochloride to lyse cells and degrade protein, than DNA in
chaotropic salt is bonded to glass fiber matrix of column. After
washing off the contaminants, the purified DNA is eluted by low salt
elution buffer or water. The entire procedure can be completed in 40
minutes without phenol/chloroform extraction and alcohol
precipitation. Expected yield of genomic DNA is up to 50 mg and
purified DNA with approximately 20-30 kb is suitable for PCR or
other enzymatic reactions.
Viral Nucleic Acid
Extraction Kit is especially designed for purification of viral RNA
or DNA from cell-free samples. The method uses detergents and a
chaotropic salt to lyse virus, than nucleic acid in chaotropic salt
is bonded to glass fiber matrix of column. Carrier RNA enhances
recovery of viral RNA in low-titer samples. After washing off the
contaminants, the purified nucleic acid is eluted by RNase-free
water. The entire procedure can be completed in 20 minutes and the
purified nucleic acid is ready for RT-PCR and PCR.
100 bp DNA
Ladder
The 100 bp DNA
Ladder containing 12 double-stranded DNA fragments are suitable for
sizing DNA from 100 to 3,000 bp. The 500 and 1,000 bp banding
pattern is 2 times brighter than the other bands on ethidium
bromide-stained agarose gels and serves as indicator.
Features
- Range: 100-3000
base pairs
- Number of Bands: 12
- Quantity: 50 mg
- Concentration: 0.1 mg / ml
- Storage Buffer: 10mM Tris-HCl(pH 8.0), 50 mM NaCl, 1.0 mM EDTA.
- Recommended loading: 0.5 mg (5 ml)
1 kb DNA Ladder
The 1 kb DNA Ladder
containing 13 double-stranded DNA fragments are suitable for sizing
DNA from 250 to 10,000 bp. The 1,000 and 3,000 bp banding pattern is
2 times brighter than the other bands on ethidium bromide-stained
agarose gels and serves as indicator.
Features
Range: 250-10,000
base pairs
Number of Bands: 13
Quantity: 50 mg
Concentration: 0.1 mg / ml
Storage Buffer: 10mM Tris-HCl(pH 8.0), 50 mM NaCl, 1.0 mM EDTA.
Recommended loading: 0.5 mg (5 ml)
